Composite
Part:BBa_K2738104
Designed by: Darshak Bhatt Group: iGEM18_Paris_Bettencourt (2018-10-10)
Enterocin fused to N-ter designed protein lyase cage monomer
Part: BBa_K2738104 (designed protein lyase cage-enterocin A)
Description We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.
The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 743
- 1000COMPATIBLE WITH RFC[1000]
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